Glytube: a conical tube and parafilm M-based method as a simplified device to artificially blood-feed the dengue vector mosquito, Aedes aegypti.

Glytube: a conical tube and parafilm M-based method as a simplified device to artificially blood-feed the dengue vector mosquito, Aedes aegypti.

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Aedes aegypti, the main vector of dengue virus, requires a blood meal to produce eggs.Although live animals are still the main blood source for laboratory colonies, many artificial feeders are available.These feeders are color touch 7/97 also the best method for experimental oral infection of Ae.aegypti with Dengue viruses.However, most of them are expensive or laborious to construct.

Based on principle of Rutledge-type feeder, a conventional conical tube, glycerol and Parafilm-M were used to develop a simple in-house feeder device.The blood feeding efficiency of this apparatus was compared to a live blood source, mice, and galaxy harmony nc no significant differences (p = 0.1189) were observed between artificial-fed (51.3% of engorgement) and mice-fed groups (40.6%).

Thus, an easy to assemble and cost-effective artificial feeder, designated "Glytube" was developed in this report.This simple and efficient feeding device can be built with common laboratory materials for research on Ae.aegypti.